A histopathological comparison of prophylactic effects of Rosmarinic Acid and Oleanolic Acid isolated from Salvia species (sage) in scopolamine induced dementia model

Objective: Alzheimer's disease (AD) is the most common form of dementia, can be created in experimental models by using toxins. Possible therapeutic effects secondary metabolites Rosmarinic (RA) and Oleanolic acids (OA) obtained from Salvia species (sage) were investigated histopathological immunohistochemical tecniques Alzheimer's-like dementia model induced scopolamine.
 Study Design: Male BALB/c mice (n: 48) 3-4 weeks old, divided into 6 groups; control received only saline(i.p.) for 21 days, scopolamine group 3 mg/kg (i.p.) between 8-21th days. Scopolamine + RA 5 days 0 OA 0-21 0-21th Animals sacrificed under anesthesia, brain tissues excised placed 10% formaldehyde, embedded paraffin wax. µm sections cut, stained examinations. Immunoreactivity Beta-amyloid accumulation was observed. ImageJ 153 software used to analyze IHC figures. Positive signaling DAB density calculated, ANOVA test with post hoc dunnett's or tukey applied, p<0.05 considered statistically significant.
 Results: In scopolamine+RA treated group, tissue degeneration less compared group. Scopolamine+OA revealed signs pyknosis neurons. The amyloid beta immunoexpression positive partially scopolamine+OA but, negative Intensity signal increased (p=0.0102). reduced Scopolamine+RA groups, significant (p=0.0281 p=0.0362, respectively).
 Conclusion: We suggest that pretreatment decreased formation ameliorated structure further studies different methods are needed commercially available.
 Keywords: Scopolamine, acid, histopathology, Amyloid